apoptosis array kit ary009 r d systems microarray Search Results


antibodies against human apoptosis related proteins  (R&D Systems)
96
R&D Systems antibodies against human apoptosis related proteins
Figure 1. Carbon ion radiation inhibited photon resistant human NPC cell lines. (A) We did two independent experiments (Group 1 and Group2) of DNA microarray analysis and defined differential expression gene (DEG) as genes with 2 folds changes of CNE-2-RR cells compared with CNE-2 cells. Comparison of multiple biological experiments was facilitated with Venn diagram (http://www.pangloss.com/seidel/Protocols/venn.cgi). (B) KEGG pathway enrichment was performed. We found that <t>apoptosis</t> pathway (including 5 genes: TNFSF10, CYCS, PIK3R5, BCL2, ATM) was one of the enrichment pathways affected following repeated irradiation and (C) pro-apoptotic genes (TNFSF10, CYCS, PIK3R5) were down-regulation and anti-apoptotic genes (BCL2, ATM) were up-regulation in CNE-2-RR cells. (D) These results suggested that after repeated photon irradiation, NPC cells would be an apoptosis-resistant cells (named CNE-2-RR cells). (E) Dose-response curves for clonogenic survival of NPC (CNE-2 and CNE-2-RR) cells after treatment of different dose of X-ray and carbon ion.
Antibodies Against Human Apoptosis Related Proteins, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibodies against human apoptosis related proteins/product/R&D Systems
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antibodies against human apoptosis related proteins - by Bioz Stars, 2026-04
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human apoptosis array  (R&D Systems)
96
R&D Systems human apoptosis array
KEY RESOURCES TABLE
Human Apoptosis Array, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human apoptosis array/product/R&D Systems
Average 96 stars, based on 1 article reviews
human apoptosis array - by Bioz Stars, 2026-04
96/100 stars
  Buy from Supplier

Image Search Results


Figure 1. Carbon ion radiation inhibited photon resistant human NPC cell lines. (A) We did two independent experiments (Group 1 and Group2) of DNA microarray analysis and defined differential expression gene (DEG) as genes with 2 folds changes of CNE-2-RR cells compared with CNE-2 cells. Comparison of multiple biological experiments was facilitated with Venn diagram (http://www.pangloss.com/seidel/Protocols/venn.cgi). (B) KEGG pathway enrichment was performed. We found that apoptosis pathway (including 5 genes: TNFSF10, CYCS, PIK3R5, BCL2, ATM) was one of the enrichment pathways affected following repeated irradiation and (C) pro-apoptotic genes (TNFSF10, CYCS, PIK3R5) were down-regulation and anti-apoptotic genes (BCL2, ATM) were up-regulation in CNE-2-RR cells. (D) These results suggested that after repeated photon irradiation, NPC cells would be an apoptosis-resistant cells (named CNE-2-RR cells). (E) Dose-response curves for clonogenic survival of NPC (CNE-2 and CNE-2-RR) cells after treatment of different dose of X-ray and carbon ion.

Journal: Journal of Cancer

Article Title: Carbon ion triggered immunogenic necroptosis of nasopharyngeal carcinoma cells involving necroptotic inhibitor BCL-x.

doi: 10.7150/jca.46316

Figure Lengend Snippet: Figure 1. Carbon ion radiation inhibited photon resistant human NPC cell lines. (A) We did two independent experiments (Group 1 and Group2) of DNA microarray analysis and defined differential expression gene (DEG) as genes with 2 folds changes of CNE-2-RR cells compared with CNE-2 cells. Comparison of multiple biological experiments was facilitated with Venn diagram (http://www.pangloss.com/seidel/Protocols/venn.cgi). (B) KEGG pathway enrichment was performed. We found that apoptosis pathway (including 5 genes: TNFSF10, CYCS, PIK3R5, BCL2, ATM) was one of the enrichment pathways affected following repeated irradiation and (C) pro-apoptotic genes (TNFSF10, CYCS, PIK3R5) were down-regulation and anti-apoptotic genes (BCL2, ATM) were up-regulation in CNE-2-RR cells. (D) These results suggested that after repeated photon irradiation, NPC cells would be an apoptosis-resistant cells (named CNE-2-RR cells). (E) Dose-response curves for clonogenic survival of NPC (CNE-2 and CNE-2-RR) cells after treatment of different dose of X-ray and carbon ion.

Article Snippet: Cell lysates obtained at 48 hours after irradiation (100 μg protein), were incubated with an array of antibodies against human apoptosis related proteins (ARY009; R&D Systems) and then were processed as per the manufacturer’s protocol.

Techniques: Microarray, Quantitative Proteomics, Comparison, Irradiation

Figure 2. Carbon ion radiation induced delayed DNA damage repair, cell cycle arrest, cytogenetic damage, morphological change and cell necrosis, indicating the possibility of necroptosis. (A) CNE-2 and CNE-2-RR cells DNA damage repair at 24 hours following X-ray or carbon-ion exposure demonstrated by fluorescence imaging of γ-H2AX loci. Original magnification: 200 ×. (B) Fluorescence micrographs of Hoechst 33342 stained cells showing micronuclei, buds, bridges. And induced fraction (%) of cytogenetic damage (cytome) at 24 hours and 48 hours after carbon ion irradiation. Original magnification: 200 ×. (C) Percentage of cells in the G2/M (mean ± SEM) phases at 24 and 48 hours following exposure to carbon beams. *P≤0.05, **P≤0.01, ***P≤0.001, ****P≤0.0001. (D) Light microscopic images showing typical morphological features of necroptosis (flattened and enlarged cells: marked by arrows) in carbon irradiated cells. Original magnification: 200 ×. (E) Bivariate plots of Annexin-V and PI in CNE-2 and CNE-2-RR cells at 48 hours following carbon ion irradiation (Q2: necrosis; Q3: apoptosis). (F). Mean value of fractions of carbon ion-induced apoptotic (Annexin+ cells) and necrotic (including necroptosis) (PI+) cell death at 48 hours. *P≤0.05, **P≤0.01, ***P≤0.001, ****P≤0.0001.

Journal: Journal of Cancer

Article Title: Carbon ion triggered immunogenic necroptosis of nasopharyngeal carcinoma cells involving necroptotic inhibitor BCL-x.

doi: 10.7150/jca.46316

Figure Lengend Snippet: Figure 2. Carbon ion radiation induced delayed DNA damage repair, cell cycle arrest, cytogenetic damage, morphological change and cell necrosis, indicating the possibility of necroptosis. (A) CNE-2 and CNE-2-RR cells DNA damage repair at 24 hours following X-ray or carbon-ion exposure demonstrated by fluorescence imaging of γ-H2AX loci. Original magnification: 200 ×. (B) Fluorescence micrographs of Hoechst 33342 stained cells showing micronuclei, buds, bridges. And induced fraction (%) of cytogenetic damage (cytome) at 24 hours and 48 hours after carbon ion irradiation. Original magnification: 200 ×. (C) Percentage of cells in the G2/M (mean ± SEM) phases at 24 and 48 hours following exposure to carbon beams. *P≤0.05, **P≤0.01, ***P≤0.001, ****P≤0.0001. (D) Light microscopic images showing typical morphological features of necroptosis (flattened and enlarged cells: marked by arrows) in carbon irradiated cells. Original magnification: 200 ×. (E) Bivariate plots of Annexin-V and PI in CNE-2 and CNE-2-RR cells at 48 hours following carbon ion irradiation (Q2: necrosis; Q3: apoptosis). (F). Mean value of fractions of carbon ion-induced apoptotic (Annexin+ cells) and necrotic (including necroptosis) (PI+) cell death at 48 hours. *P≤0.05, **P≤0.01, ***P≤0.001, ****P≤0.0001.

Article Snippet: Cell lysates obtained at 48 hours after irradiation (100 μg protein), were incubated with an array of antibodies against human apoptosis related proteins (ARY009; R&D Systems) and then were processed as per the manufacturer’s protocol.

Techniques: Fluorescence, Imaging, Staining, Irradiation

KEY RESOURCES TABLE

Journal: Cell reports

Article Title: RNA-binding protein FXR1 drives cMYC translation by recruiting eIF4F complex to the translation start site

doi: 10.1016/j.celrep.2021.109934

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: A human apoptosis array (Proteome Profiler Cat# ARY009; R&D Systems) was used to analyze apoptosis-related protein profiles according to manufacturer instructions.

Techniques: Control, Recombinant, Modification, Transfection, Protease Inhibitor, CCK-8 Assay, Bicinchoninic Acid Protein Assay, cDNA Synthesis, SYBR Green Assay, Caspase-Glo Assay, Immunoprecipitation, Plasmid Preparation, Staining, In Situ, Microarray, Western Blot, Expressing, Labeling, Negative Control, Mutagenesis, Software